ATP-dependent Rotation of Mutant ATP Synthases Defective in Proton Transport
نویسندگان
چکیده
منابع مشابه
ATP synthesis driven by proton transport in F1F0-ATP synthase.
Topical questions in ATP synthase research are: (1) how do protons cause subunit rotation and how does rotation generate ATP synthesis from ADP+Pi? (2) How does hydrolysis of ATP generate subunit rotation and how does rotation bring about uphill transport of protons? The finding that ATP synthase is not just an enzyme but rather a unique nanomotor is attracting a diverse group of researchers ke...
متن کاملThe proton conducting F0-part of bacterial ATP synthases.
2. Inhibitor-resistant mutants ... 0 • • •• • • •••• •• • •• 0 0 0 0 0 0 •• • • • 0 ••• 0 0 •• 0 0 •••• 0 •• 0 0 0 0 0 0 •• 0 ••• 0 0 0 0 4 4 6 6 9 10 11 11 12 13 13 15 V Functional aspects ...... 0 • ••••• 0 0 • 0 •••• 0 • 0 0 • 0 •• • 0 0 0 • 0 0 • • ••••• 0 0 0 ••••••• • •• 0 0 0 0 0 0 •• ••••• 0 0 0 • 16 A. Evolutionaryaspects .... 0 0 ••• • ••••••• 0 ••••• ••••• • ••••••••••••••• ••••• • •...
متن کاملEssentials for ATP synthesis by F1F0 ATP synthases.
The majority of cellular energy in the form of adenosine triphosphate (ATP) is synthesized by the ubiquitous F(1)F(0) ATP synthase. Power for ATP synthesis derives from an electrochemical proton (or Na(+)) gradient, which drives rotation of membranous F(0) motor components. Efficient rotation not only requires a significant driving force (DeltamuH(+)), consisting of membrane potential (Deltapsi...
متن کاملATP-dependent lysosomal cystine efflux is defective in cystinosis.
Lysosomes containing large amounts of the amino acid, cystine, were obtained from transformed, cultured, human lymphoblasts which had been exposed to cystine dimethyl ester. Lysosomal cystine efflux was greatly enhanced by exogenous ATP in cell lines from normal individuals. Cystine efflux was unresponsive to ATP in lysosomes from individuals with the disorder, cystinosis. Efflux of cystine fro...
متن کاملPreparation of Membrane Vesicles Enriched in ATP-Dependent Proton Transport from Suspension Cultures of Tomato Cells.
Membranes enriched in ATP-dependent proton transport were prepared from suspension cultures of tomato cells (Lycopersicon esculentum Mill cv VF36). Suspension cultures were a source of large quantities of membranes from rapidly growing, undifferentiated cells. Proton transport activity was assayed as quench of acridine orange fluorescence. The activity of the proton translocating ATPase and of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2005
ISSN: 0021-9258
DOI: 10.1074/jbc.m502650200